This book presents an advanced and integrated treatment of chromatographic and electrophoretic techniques, emphasising their theoretical foundations and practical application in modern chemical and biological analysis. It explains the physical, chemical, and mathematical principles governing separation processes, transport phenomena, and measurement systems. The discussion links molecular behaviour with analytical performance, addressing factors that influence resolution, sensitivity, and reliability. Attention is given to thermal, electrical, and diffusive processes that underpin analytical instrumentation and experimental design. By connecting fundamental laws with laboratory practice, the book enables readers to interpret analytical data critically, optimise methodologies, and understand the limitations of separation techniques within research and industrial contexts, and supports advanced study, interdisciplinary research, and methodological innovation across chemistry, biotechnology, materials science, and applied physics domains in contemporary laboratories worldwide.

It is intended for postgraduate students, researchers, and professionals in analytical chemistry and related disciplines. The book serves as an academic reference and practical guide, supporting experimental design, data interpretation, and advanced training in separation science for research, quality control, and industrial laboratories within academic institutions, regulatory settings, and technology-driven analytical environments globally today.